Present‐Day DNA Contamination in Ancient DNA Datasets
نویسندگان
چکیده
منابع مشابه
Ancient DNA from Human and Animal
Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...
متن کاملAncient DNA
Ancient DNA is a field of molecular evolutionary biology that usesDNA sequence data recovered frompoorly preserved organisms, usually deceased for hundreds to hundreds of thousands of years. Ancient DNA data can provide unique snapshots in time to better understand how populations and species evolve. The field was born in the early 1980s,when thefirst ancientDNAsequences were recovered from pre...
متن کاملMinimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples: application to ancient DNA studies.
PCR analyses of ancient and degraded DNA suffer from their extreme sensitivity to contamination by modern DNA originating, in particular, from carryover contamination with previously amplified or cloned material. Any strategy for limiting carryover contamination would also have to be compatible with the particular requirements of ancient DNA analyses. These include the need (i) to amplify short...
متن کاملEpigenetics of Ancient DNA
Initially, the study of DNA isolated from ancient specimens had been based on the analysis of the primary nucleotide sequence. This approach has allowed researchers to study the evolutionary changes that occur in different populations and determine the influence of the environment on genetic selection. However, the improvement of methodological approaches to genome-wide analysis has opened up n...
متن کاملAdvances in ancient DNA studies
The first aDNA studies used bacterial cloning to amplify small sequences retrieved from skins of animal and human mummies, and revealed the inefficient reaction kinetics of this technique (Higuchi et al., 1984; Pääbo 1985, 1989). These studies demonstrated that the genetic material surviving in ancient specimens was often principally microbial or fungal in origin. Endogenous DNA was generally l...
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ژورنال
عنوان ژورنال: BioEssays
سال: 2020
ISSN: 0265-9247,1521-1878
DOI: 10.1002/bies.202000081